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Three different mechanisms (gene expression, angiogenesis, cell migration) engaging three complementary biological pathways

The mechanism involves inhibition of nitric oxide synthase (NOS) activity: glutathione binds nitric oxide (NO), a critical neurotransmitter in gut motility, effectively sequestering it before it can signal smooth muscle relaxation and coordinated peristaltic waves

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We furthermore confirmed by mass spectrometry (MS) that the reaction of GSSH with either ferric cyt c or ABTS radicals indeed generates GSSSSG (Fig